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染色体畸变分析为肿瘤细胞遗传学研究的常规方法。近来,有研究表明癌细胞长期培养并不会引起额外特征性染色体改变。PG 癌系为我室建立的具有高转移性人肺癌细胞系,在体外已传代数年。最近,我们从 PG母系中分离出多个亚克隆,并具有相同的生物学特性。为探讨其遗传稳定性及进一步研究分子遗传改变与生物学行为的关系,我们比较了 PG 母系与两个亚克隆的染色体畸变。结果表明:1,3,6,7,11,13,17,19号染色体异常与其它肺癌的研究报道相近;母系与亚系的染色体畸变类型几乎一致。因此说明 PG 细胞在长期培养过程中,其遗传特性是稳定的,并相似于一般肺癌遗传学改变。
Chromosome aberration analysis is a routine method for the study of tumor cytogenetics. Recently, studies have shown that long-term culture of cancer cells does not cause additional characteristic chromosome changes. The PG cancer line is a highly metastatic human lung cancer cell line established in our department and has been passed on in vitro for several years. Recently, we isolated multiple subclones from the PG maternal line and shared the same biological characteristics. In order to investigate its genetic stability and further study the relationship between molecular genetic alterations and biological behavior, we compared chromosomal aberrations of the PG maternal line with two subclones. The results showed that the abnormalities of chromosomes 1, 3, 6, 7, 11, 13, 17 and 19 were similar to those of other lung cancers; the maternal and subline chromosomal aberrations were almost identical. Therefore, the genetic characteristics of PG cells in the long-term culture process are stable and similar to the general genetic changes in lung cancer.