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目的探讨乙苯对大鼠肾小管上皮细胞氧化损伤及转录调控因子核因子E2相关因子2(Nrf2)、血红素加氧酶Ⅰ型(HO-1)表达的影响。方法 NRK-52e细胞分别用0、30、60、90μmol/L乙苯染毒24 h,染毒结束后分别测定细胞外乳酸脱氢酶(LDH)活力和细胞内活性氧(ROS)水平、丙二醛(MDA)含量及过氧化氢酶(CAT)活力,以Western blot检测NRK-52e细胞Nrf2、HO-1的表达。结果与对照组相比,60、90μmol/L剂量组细胞外LDH和细胞内CAT活力均增高(P<0.05),90μmol/L剂量组细胞内MDA含量高于对照组(P<0.05),各剂量组细胞内二氯荧光黄(DCF)荧光强度升高(P<0.05),60及90μmol/L剂量组HO-1和Nrf2表达水平高于对照组和30μmol/L剂量组(P<0.05)。结论乙苯可导致细胞氧化损伤,并且通过激活Nrf2,诱导HO-1表达。
Objective To investigate the effect of ethylbenzene on the oxidative injury of renal tubular epithelial cells and the expression of nuclear factor E2 (NF2) and heme oxygenase type 1 (HO-1) in rats. Methods NRK-52e cells were exposed to 0, 30, 60 and 90μmol / L ethylbenzene for 24 hours. After lactation, the activities of extracellular lactate dehydrogenase (LDH) and intracellular reactive oxygen species (ROS) Dialdehyde (MDA) content and catalase (CAT) activity. The expression of Nrf2 and HO-1 in NRK-52e cells were detected by Western blot. Results Compared with the control group, the levels of extracellular LDH and intracellular CAT in 60 and 90μmol / L groups were significantly increased (P <0.05), and the levels in 90μmol / L group were higher than those in control group (P <0.05) (P <0.05). The expression of HO-1 and Nrf2 in the 60 and 90μmol / L groups were higher than those in the control and 30μmol / L groups (P <0.05) . Conclusion Ethylbenzene can lead to cell oxidative damage and induce HO-1 expression by activating Nrf2.