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二氢青蒿素与鲁米诺在碱性条件下不需过氧化氢就能直接发生化学发光反应，氯化血红素对这一新的体系同样具有强烈的催化作用，运用这一新的发光体系建立了灵敏度高、特异性强、操作方便的测定抗疟新药二氢青蒿素的化学发光新方法，线性范围为０～３０００ｎｇ／ｍｌ，线性方程为Ｙ＝１．１４６Ｘ＋２．９３（０～１００ｎｇ／ｍｌ），ｒ＝０．９９４；Ｙ＝２．１７Ｘ－１３９（１００～３０００ｎｇ／ｍｌ），ｒ＝０．９９９，检出限为１．４ｎｇ／ｍｌ，变异系数为３．３％。文中提示二氢青蒿素中的过氧键是鲁米诺发光反应所需的超氧自由基的来源。
Dihydroartemisinol and luminol do not require hydrogen peroxide in alkaline conditions can be directly chemiluminescence reaction, hemin for this new system also has a strong catalytic role, the use of this new light A new chemiluminescence method was developed for the determination of dihydroartemisinin, a new antimalarial drug with high sensitivity, specificity and ease of operation. The linear range was 0-3000 ng / ml. The linear equation was Y = 1.146X + 2.93 (0 ~ 100 ng / ml), r = 0.994; Y = 2.17X-139 (100-3000 ng / ml), r = 0.999. The limit of detection was 1.4 ng / ml and the coefficient of variation was 3.3%. It is suggested that the peroxy bond in dihydroartemisinin is the source of superoxide radical required by luminol luminescence reaction.