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【目的】为了发现新的农作物病原菌抗菌肽,人工设计并构建了大容量短肽文库,从中筛选并合成96条短肽用于鉴定其对农作物病原菌的抑菌活性。【方法】采用琼脂扩散法,对靶标菌—棉花枯萎病菌(Fusarium f.sp.vasinfecum)、棉花红腐病菌(Fusarium moniliforme)、小麦根腐病菌(Bipolaris sorokiniana)和马铃薯早疫病菌(Alternaria solani)进行抑菌初筛,并测定了有抗菌作用短肽的最小抑菌浓度和抑菌持久性。【结果】得到了A6、D4和F10对上述四种病原真菌抑菌效果较强,抑菌时间较长的抗菌肽,通过与抗菌肽数据库氨基酸序列对比,未见这3条抗菌肽的同源序列。【结论】研制的3条短肽属于新颖抗菌肽,为防治农作物真菌病害提供了新的基因资源。
【Objective】 In order to find new antibacterial peptides of crop pathogens, we designed and constructed a large-capacity short peptide library, screening and synthesizing 96 short peptides to identify their antibacterial activity against the pathogen of crops. 【Method】 The target bacteria, Fusarium f.sp. vasinfecum, Fusarium moniliforme, Bipolaris sorokiniana and Alternaria solani, were isolated by agar diffusion method. The results showed that the antibacterial short peptides had the minimum inhibitory concentration and the bacteriostasis persistence. 【Result】 The results showed that the antibacterial peptides A6, D4 and F10 had stronger antibacterial activity and longer antibacterial activity against the above four pathogenic fungi. Compared with the amino acid sequence of the antibacterial peptide database, no homology of the three antibacterial peptides was found sequence. 【Conclusion】 The three short peptides developed were novel antibacterial peptides, which provided new genetic resources for the control of fungal diseases in crops.