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本试验选用薮北种不定胚发育形成的芽.先在0.75％琼脂.3.0%蔗糖,1/2MS无机养分的培养基中进行1年以上的继代培养(添加IBAO.1mg/L、BA1.0 mg/L)。然后剪取带腋芽的节作外植体供试.试验用培养基均为0.75％琼
In this experiment, buds formed by the development of adventitious embryos were selected and subcultured for more than 1 year in medium with 0.75% agar, 3.0% sucrose and 1 / 2MS inorganic nutrient, and then added with 1 mg / L IBAO. 0 mg / L). Then cut the section with axillary buds as explants for testing.Experimental media were 0.75% agar