In vitro Recombination and Identification of Mutated Fragment Corresponding to Regulation Region of

• 作 者: HUANG Changzheng LIN Nengxing
• 机 构: Department of Dermatology, Union Hospital, Tongji Medical College, of Huazhong University of Science
• 出 处: 华中科技大学学报（医学）（英德文版）
• 发表日期: 2007年5期
• 关键词: Neisseria gonorrhoeae genes mutation mtr drug resistance

A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria Gonorrhoeae (NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene. According to the technique of gene splicing by over-lap extension (SOEing), a DNA segment with specific mutagenesis was constructed by two-step po-lymerase chain reaction (PCR). The mutation fragments EF could be used for the next experiment in which the mutation NG strains were induced. By comparing the recombinant EF fragments to the corresponding DNA fragments of clinical NG strains, 2 of these were not compatible completely. The results of sequencing revealed that there was a 9 bp deletion between the 45 to 54 inverted repeat se-quence localized within the mtrR promoter. It can be confirmed that the fragments EF are the specifi-cally designed mutant fragments.