脐血与骨髓间充质干细胞的成骨细胞定向诱导及其成骨活性比较

• 作 者: 程志安 周敦华 宋少云 吴燕峰 沈慧勇 曾志勇 刘尚礼
• 机 构: 中山大学附属第二医院骨科,中山大学附属第二医院儿科,中山大学生物防治国家重点实验室,中山大学附属第二医院脐血库,中山大学附属第二医院骨科,中山大学附属第二医院消化室,中山大学附属第二医院骨科 广东省广
• 出 处: 中国临床康复
• 发表日期: 2004年35期
• 关键词: 间充质干细胞 脐血 成骨细胞

目的:研究脐血与成人骨髓间充质干细胞(mesenchymalstemcells,MSCs)的定向分化为成骨细胞的条件及其成骨活性,比较两种不同MSCs定向成骨细胞诱导后的成骨活性。方法:采用标准Ficoll-Hypaque技术分离脐血和成人骨髓MSCs,以地塞米松、β-磷酸甘油和维生素C为辅剂定向诱导成骨细胞,碱性磷酸酶、骨矿化结节和I型胶原作为成骨细胞鉴定与活性评价的指标。结果:两种MSCs的细胞形态和生物学特性无明显差异。定向成骨细胞诱导后,成骨细胞标志性产物碱性磷酸酶、骨钙素、上清钙、骨矿化结节和I型胶原均得到了稳定的表达,并显著高于未诱导MSCs,而且两种细胞的成骨活性差异无显著性意义。结论:脐血和成人骨髓MSCs在适当的条件下均可向成骨细胞定向转化,两种细胞均可作为骨组织工程的种子细胞。

OBJECTIVE: To investigate the osteogenic differentiation and osteogenic activity of mesenchymal stem cells (MSCs) derived from umbilical cord blood and adult MSCs, and to compare the osteogenic activity of osteoblasts induced by two different MSCs. Methods: Cord blood and adult bone marrow MSCs were isolated by standard Ficoll-Hypaque technique. Osteoblasts, alkaline phosphatase, bone mineralization nodules and type I were induced by dexamethasone, β-phosphoglycerol and vitamin C Collagen as an indicator of osteoblast identification and activity evaluation. Results: The morphological and biological characteristics of the two MSCs showed no significant difference. Osteoblast-like products alkaline phosphatase, osteocalcin, supernatant calcium, bone mineralization nodules and type I collagen were all expressed stably after induced osteoblast induction, and were significantly higher than that of non-induced MSCs. There was no significant difference in osteogenic activity between the two cells. CONCLUSION: Cord blood and adult bone marrow MSCs can be transformed into osteoblasts under appropriate conditions. Both of them can be used as seed cells for bone tissue engineering.