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吡喹酮是当前治疗人体血吸虫病的首选药物,它对人感染的3种血吸虫的成虫都非常有效。本文报道了该药治疗感染曼氏血吸虫的小鼠后肺内幼虫表面抗原的变化和由虫体的酶引起肺出血的情况。在实验室内,以光滑双脐螺和金色仓鼠传代保存的波多黎各株曼氏血吸虫尾蚴接种小鼠,每鼠6000条,感染后6天用以0.15M氯化钠溶解的吡喹酮液0.1ml注入小鼠的腿肌。3组小鼠的用药剂量分别为0、50mg/kg和200mg/kg。给药后1小时剖杀小鼠,用含0.5％乳清蛋白水解物(Elac)和10u/ml肝素的伊氏平衡盐水灌肺并将肺剪碎用Elac在37℃内孵育3小时后过筛,以1200rpm离心2分钟分离幼
Praziquantel is currently the drug of choice for the treatment of human schistosomiasis and is very effective against adults infected with all three human schistosomes. This article reports the changes of surface antigens in the larvae of the lungs of mice infected with Schistosoma mansoni and the pulmonary hemorrhage caused by the enzymes of the parasites. Mice were vaccinated with S. cercariae of Schistosoma mansoni, which were passaged and deposited in the laboratory for two generations of smooth-bodied umbilical snails and golden hamsters, 6,000 animals per mouse for 6 days with 0.1 ml of praziquantel dissolved in 0.15 M sodium chloride Into the mouse leg muscle. The dosage of the three groups of mice was 0, 50 mg / kg and 200 mg / kg, respectively. Mice were sacrificed one hour after dosing and the lungs were irrigated with ethio-equilibrated saline containing 0.5% whey protein hydrolyzate (Elac) and 10 u / ml heparin and lungs were cut with Elac for 3 hours at 37 ° C Sieve and centrifuge at 1200 rpm for 2 minutes to isolate young