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通络醒脑泡腾片(TLXNET)是源自古方芎归汤加减而来的专利处方,具有改善认知功能的作用,然而关于它对Aβ25-35海马注射痴呆模型大鼠的尿液代谢产物影响及抗痴呆作用并不明确。该实验以Aβ25-35海马注射痴呆大鼠模型为研究对象,采用代谢组学技术研究空白大鼠、Aβ25-35海马注射痴呆模型大鼠和通络醒脑泡腾片干预大鼠尿液中代谢物组时量的轨迹变化和对应关系,确定其特征性代谢标志物,并基于此标志物的代谢轨迹变化阐明通络醒脑泡腾片的抗痴呆作用。实验结果初步确定了吲哚-5,6-醌、4-羟基苯基丙酮酸(4-HPPA)、皮质醇和3-硫代乳酸等4个特征性代谢标志物,它们主要参与体内多巴胺神经系统、糖皮质激素及能量代谢途径,且通络醒脑泡腾片对4个内源性生物标记物代谢轨迹的扰动具有明显的回调作用。该实验表明Aβ25-35海马注射痴呆大鼠模型有明确的尿液代谢特征性内源性生物标志物,通络醒脑泡腾片可以通过回调其代谢轨迹的扰动而达到抗痴呆的作用。
Tongluo Xingnao effervescent tablets (TLXNET) is a patent prescription derived from ancient Xionggui Decoction, which has the effect of improving cognitive function. However, its metabolites of urine in dexamethasone model rats injected with Aβ25-35 hippocampus Effect and anti-dementia role is not clear. In this experiment, the rat model of dementia was injected with Aβ25-35 into the hippocampus of rats. Metabolomics was used to study the metabolism of urine in the blank rats, Aβ25-35 hippocampus dementia model rats and Tongluo Xingnao effervescent tablet Determine the characteristic metabolic markers of the metabolites, and determine the anti-dementia effects of Tongluo Xingnao effervescent tablets based on the metabolic pathways of the markers. The experimental results initially identified four characteristic metabolic markers of indole-5,6-quinone, 4-hydroxyphenylpyruvate (4-HPPA), cortisol and 3-thiolactic acid, which are mainly involved in the dopamine nervous system , Glucocorticoids and energy metabolism pathways, and Tongluo Xingnao effervescent tablet has obvious callback effect on the disturbance of four endogenous biomarker metabolic loci. The experiment showed that Aβ25-35 hippocampus dementia rat model has a clear endogenous biomarkers characteristic of urinary metabolites, Tongluo Xingnao effervescent tablets by the metabolic path of the callback perturbation to achieve the role of anti-dementia.