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本工作用与视束相连的仓鼠上丘离体薄片进行细胞内记录研究。受试的８５个上丘浅层细胞中，６７个细胞由刺激视束引发的兴奋性突触后电位（ＥＰＳＰ）的高度在５－ＨＴ灌流时，从平均对照７．８±２．１ｍＶ降至２．７±１．９ｍＶ，膜电位和输入阻抗无明显变化，５－ＨＴ上述作用与５－ＨＴ１Ｂ受体激动剂ＴＦＭＰＰ与ＣＧＳ－１２０６６Ｂ的效应类似，为５－ＨＴ１Ａ／１Ｂ受体拮抗剂ｐｉｎｄｏｌｏｌ所阻断，不受其它５－ＨＴ受体拮抗剂ｓｐｉｐｅｒｏｎｅ（５－ＨＴ１Ａ、１ｃ／５－ＨＴ＿２）、ＮＡＮ－１９０（５－ＨＴ＿２）及ＭＤＬ－７２２２２（５－ＨＴ＿３）所影响。在克氏液及低钙高镁液中，５－ＨＴ不降低压力注射ｇｌｕｔａｍａｔｅ引致的膜去极反应。上述结果提示，５－ＨＴ经突触前１Ｂ受体抑制视束至上丘浅层神经元的兴奋传递。
This work with the light beam attached to the hamstring on the off-thin sheet for intracellular recording studies. Of 85 supernantal superficial cells tested, the height of excitatory postsynaptic potential (EPSP) of 67 cells stimulated by the stimulating optic tract decreased from 7.8 ± 2.1 mV in the mean control during 5-HT perfusion To 2.7 ± 1.9mV, there was no significant change in membrane potential and input impedance. The above effect of 5-HT was similar to the effect of 5-HT1B receptor agonist TFMPP and CGS-12066B, and was a 5-HT1A / 1B receptor antagonist pindolol, and was not affected by other 5-HT receptor antagonists spiperone (5-HT1A, 1c / 5-HT_2), NAN-190 (5-HT_2) and MDL-72222 (5-HT_3). 5-HT does not reduce the depigmentation of glutamate-induced membrane desensitization in both Krebs and low calcium high magnesium fluids. These results suggest that 5-HT pre-synaptic 1B receptor inhibits the excitory transmission of the optic nerve to the superior neurons in the superior colliculus.