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目的:比较真菌特异性引物内转录片段(ITS)PCR检测方法与培养的敏感性,探讨真菌ITS PCR检测方法的敏感性与特异性及其在真菌性鼻窦炎诊断中的意义。方法:取18例临床诊断为真菌性鼻窦炎患者的鼻窦内分泌物,分别做ITS PCR及培养,比较2种方法阳性率的差异及检出真菌的相符率;同时以20例临床诊断为非真菌性慢性鼻窦炎患者鼻窦内分泌物为对照,进一步探讨ITS PCR对真菌性鼻窦炎临床诊断的意义。结果:18例真菌性鼻窦炎的标本中,ITS PCR方法检测真菌阳性的例数为14例(78%);培养法的阳性例数为8例(44%)。20例慢性鼻窦炎ITS PCR方法检测阳性例数为1例(5%)。结论:ITS PCR方法对于真菌性鼻窦炎的快速诊断很有价值,并能通过测序明确感染菌种,是一种高敏感性和高特异性的方法。
OBJECTIVE: To compare the sensitivities and specificities of ITS PCR detection methods and culture in fungi-specific primers, to explore the sensitivity and specificity of fungal ITS PCR detection and its significance in the diagnosis of fungal sinusitis. Methods: Eighteen patients with fungal rhinosinusitis were enrolled in this study. Eighteen clinically diagnosed as sinusitis endocrine patients were subjected to ITS PCR and culture, respectively. The positive rates of the two methods were compared and the coincidence rates of fungi were detected. At the same time, 20 cases of non-fungal Sinusitis patients with chronic sinusitis endocrine as a control to further explore ITS PCR fungal sinusitis clinical diagnosis significance. Results: Among 18 specimens of fungal sinusitis, 14 cases (78%) were detected by ITS PCR and 8 cases (44%) by culture method. One case (5%) was detected by ITS PCR in 20 cases of chronic sinusitis. CONCLUSION: ITS PCR is valuable for the rapid diagnosis of fungal sinusitis. It is a highly sensitive and specific method to identify bacterial species by sequencing.