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提出了一种基于酶催化沉积放大检测芜菁花叶病毒(Turnip mosaic virus,TuMV)的生物传感技术。该方法先通过夹心免疫反应,将碱性磷酸酯酶标记的芜菁花叶病毒抗体固定到电极表面,然后通过碱性磷酸酯酶催化还原银离子在电极表面形成不溶性沉积物,从而放大电化学检测信号。考察了抗体的用量和沉积时间对免疫分析的影响,结果显示传感器信号响应与芜菁花叶病毒原液的浓度在10~1000倍稀释范围内呈良好的线性关系,检出限达到500000倍芜菁花叶病毒原液稀释浓度。
A biosensing technology based on enzyme-catalyzed deposition amplification detection of turnip mosaic virus (TuMV) was proposed. The method comprises the following steps of: sandwiching immune reaction, fixing alkaline phosphatase-labeled turnip mosaic virus antibody on the surface of the electrode, and then catalyzing the reduction of silver ions by alkaline phosphatase to form insoluble deposits on the electrode surface so as to amplify the electrochemical Detection signal. The effects of the amount of antibody and deposition time on the immunoassay were investigated. The results showed that there was a good linear relationship between the signal response of the sensor and the concentration of the turnip mosaic virus solution in the range of 10-1000 dilutions, with the detection limit of 500000 times Mosaic virus stock solution concentration.