Cloning and Sequence Analysis of Mutant OmpF from Antibiotic Resistant Escherichia coli

• 作 者: Xin NIE Li ZHANG Tingting LIU
• 机 构: SchoolofChemistryandPharmaceuticalEngineering
• 出 处: 农业生物技术：英文版
• 发表日期: 2014年3期
• 关键词: mOmpF Outer MEMBRANE PROTEIN OMPF Antibiotic resistance mOmpF Outer membrane pro
• 基金项目: Supported by the National Natural Science Foundation of China（No.31100089）,Scientific Research Foundation of the Education Department of Sichuan Province（No.11ZB102）,the Talent Project of Sichuan Univ

[Objective]This study aimed to investigate the mutations of OmpF from an isolated antibiotic resistant Escherichia coli strain.[Methods]The mutant OmpF(mOmpF)from antibiotic resistant E.coli was amplified by PCR with Pfu DNA polymerase and ligated into th