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目的研究依非韦仑(EFV)的血浆浓度与细胞色素P4502B6(CYP2B6)基因多态性的关系。方法用高效液相色谱仪(HPLC系统)测定深圳市东湖医院艾滋病专科门诊68例患者,服用EFV后8-12小时的EFV血浆浓度,用逆转录-聚合酶链反应检测所有患者的CYP2B6 516位点的G>T基因频率。结果CYP2B6 516位点的基因型39例是GG型,26例是GT型,3例是TT型。各组EFV的血浆浓度分别是(3.3±1.2)μg/ml、(4.7±2.3)μg/ml、(9.0±0.8)μg/ml,其差异有显著的统计学意义(P<0.01)。结论GT基因型频率在中国人相当常见,该基因型可出现较高的EFV血浆浓度;TT基因型虽然少见,但EFV血浆浓度异常升高。检测CYP2B6基因多态性可帮助临床个性化EFV用量。
Objective To study the relationship between plasma concentration of efavirenz (EFV) and the polymorphism of cytochrome P4502B6 (CYP2B6) gene. Methods Sixty - eight patients with HIV / AIDS in Donghu Hospital of Shenzhen City were enrolled in this study. Plasma concentrations of EFV 8-12 hours after taking EFV were measured. All patients were tested for CYP2B616 using reverse transcriptase - polymerase chain reaction Point G> T gene frequency. Results CYP2B6 516 loci genotype 39 cases are GG type, 26 cases are GT type, 3 cases are TT type. The plasma concentrations of EFV in each group were (3.3 ± 1.2) μg / ml, (4.7 ± 2.3) μg / ml and (9.0 ± 0.8) μg / ml, respectively. The difference was statistically significant (P <0.01). Conclusion GT genotype frequency is quite common in Chinese people, and this genotype may have higher EFV plasma concentration. Although TT genotype is rare, EFV plasma concentration is abnormally elevated. Detecting CYP2B6 gene polymorphisms can help clinicalize the use of EFV.