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调查并分离了广东、海南、广西、福建和云南等香蕉枯萎病疫区的一批病原菌样本。利用高效液相色谱—电喷雾离子阱质谱(HPLC-ESI-MS)法对所分离的28个Fusarium oxysporum f.sp.cubense菌株的次生代谢产物进行初步分析。发现了分子量为179D的镰刀菌酸及分子量约为783D的未知产物;利用1H-NMR对一株4号生理小种的未知产物进行了结构测定,结果表明该化合物为白僵菌素。离体试验表明白僵菌素能够导致香蕉假茎腐烂。
A batch of pathogen samples of banana wilt disease in Guangdong, Hainan, Guangxi, Fujian and Yunnan were investigated and isolated. The secondary metabolites of 28 isolated strains of Fusarium oxysporum f.sp.cubense were analyzed by high performance liquid chromatography-electrospray ion trap mass spectrometry (HPLC-ESI-MS). Fusarium acid with a molecular weight of 179D and unknown product with a molecular weight of about 783D were found. The structure of unknown product of a race 4 was analyzed by1H-NMR. The results showed that the compound was bensonin. In vitro tests showed that bevermectin could cause rotting of banana pseudostem.