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目的通过对2010—2014年新疆食源性致病菌分离株的复核鉴定,了解各地(州、市)监测实验室的检测质量和技术水平。方法依据国家《食品微生物学检验方法》GB 4789-2010规定的相关方法操作。结果 2010—2014年新疆各监测实验室报送分离菌株9类共计295株,鉴定符合252株,符合率85.42%;2013年符合率最低、为77.14%,2014年的符合率最高、为94.51%,5年间符合率有逐年上升的趋势,不同年份间符合率差异有统计学意义(χ2=10.42,P<0.05);3类食源性致病菌的符合率高于90.00%,符合率较高的依次为铜绿假单胞菌100.00%、单核细胞增生李斯特氏菌92.59%、沙门氏菌90.63%;符合率最低的为副溶血性弧菌60.00%。结论新疆各地(州、市)食源性致病菌监测实验室的检测能力和技术水平还有待加强,应加大对基层实验室的设备投入、基础建设和人员技能培训,提高监测实验室的检测技术水平。
OBJECTIVE To understand the quality of detection and technical level of monitoring laboratories in various places (prefectures and cities) through the review and identification of isolates of foodborne pathogens from 2010 to 2014 in Xinjiang. Methods According to the national “food microbiology test method” GB 4789-2010 provides the relevant methods of operation. Results A total of 295 strains of 9 isolates were reported from all monitoring laboratories in Xinjiang in 2010-2014. The identification coincided with 252 strains with a coincidence rate of 85.42%. The lowest coincidence rate was 77.14% in 2013 and the highest coincidence rate in 2014 was 94.51% , The coincidence rate increased year by year in five years, and the coincidence rate in different years was statistically significant (χ2 = 10.42, P <0.05). The coincidence rates of three kinds of food-borne pathogens were higher than 90.00% Pseudomonas aeruginosa 100.00%, Listeria monocytogenes 92.59% and Salmonella 90.63%, respectively. The lowest one was Vibrio parahaemolyticus 60.00%. Conclusion The testing capability and technical level of food-borne pathogenic bacteria monitoring laboratories in all parts of Xinjiang should be strengthened. Equipment input, infrastructure and personnel skills training in grassroots laboratories should be increased to improve the monitoring laboratory’s Testing technology.