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目的:研究淫羊藿苷对体外培养大鼠股骨组织的影响。方法:无菌条件下体外分离培养大鼠股骨组织,采用培养液中终浓度为1×10-5mol·L-1的淫羊藿苷进行药物干预,检测碱性磷酸酶活性(alkaline phosphatase,ALP)、钙盐沉积量以及Real-Time RT-PCR检测骨保护素(osteoprotegerin,OPG),Ⅰ-型胶原(collagen-1),Runt-related transcription factor 2(Runx-2)mRNA的表达情况。结果:1×10-5mol·L-1淫羊藿苷处理组ALP活性从第6～12天呈升高趋势,第12～18天降低;6～15 d各组钙盐沉积量呈升高趋势,第18天降低;淫羊蒮苷组处理股骨组织2～4 d时促进Runx-2 mRNA的表达水平,处理8 d时抑制Runx-2 mRNA的表达;淫羊蒮苷组处理股骨组织2～8 d时促进Collage-1 mRNA的表达水平;淫羊蒮苷组处理股骨组织2～4 d时能促进OPG mRNA的表达水平,处理6,8 d时与对照组比较无统计学意义。结论:淫羊藿苷可显著提高体外培养大鼠股骨组织中骨代谢相关基因的表达水平、增加钙盐沉积量以及ALP活性。
Objective: To study the effect of icariin on the cultured femur in vitro. METHODS: Rat femur tissue was isolated and cultured in vitro under sterile conditions. Icariin (1 × 10-5 mol·L-1) in the culture medium was used for drug intervention. Alkaline phosphatase (ALP) ), Calcium deposition and Real-time RT-PCR were used to detect the expression of osteoprotegerin (OPG), collagen-1 and Runt-related transcription factor 2 (Runx-2) mRNA. Results: The ALP activity of Icariin treated with 1 × 10-5 mol·L-1 increased from the 6th to the 12th day and decreased from the 12th to the 18th day. The calcium deposition increased from 6th to 15th day The trend was decreased on the 18th day. The expressions of Runx-2 mRNA in the femoral ianosine group were increased 2 ~ 4 days after treatment, and the expression of Runx-2 mRNA was inhibited on the 8th day. The femoral tissue 2 ~ 8 d, the expression of Collage-1 mRNA was promoted. The expressions of OPG mRNA in femoral ianosine treated group were increased 2-4 days, and there was no significant difference between the control group and the 6th and 8th day. CONCLUSION: Icariin can significantly increase the expression of bone metabolism-related genes in rat femur tissue and increase the calcium deposition and ALP activity.