论文部分内容阅读
目的研究胰岛素样生长因子-1(insulin-like growth factor-I,IGF-I)在难治性癫癎患者颞叶中的表达,探讨其对难治性癫癎的作用。方法收集难治性癫患者术后脑组织,按手术时间的先后建立难治性癫癎脑组织库,随后在脑库中按随机化原则从中抽取30例(实验组)颞叶,正常对照组为颅脑外伤手术切除的正常颞叶组织12例,采用免疫组化、western blot测定两组的IGF-I表达并进行比较。结果免疫组化结果提示IGF-1分布于神经元和胶质细胞膜,在难治性癫癎患者颞叶中的表达比对照组明显增加。western blot的结果和免疫组化结果一致。结论IGF-1在难治性癫癎患者脑组织的表达升高可能与脑的保护机制有关,这种神经营养因子的存在有利于挽救因癫癎发作而损伤的神经元。
Objective To investigate the expression of insulin-like growth factor-I (IGF-I) in the temporal lobe of refractory epilepsy patients and its effect on refractory epilepsy. Methods The brain tissues of patients with intractable epilepsy were collected. According to the principle of randomization, 30 cases (experimental group) of temporal lobe, normal control group Twelve cases of normal temporal lobe were excised for craniocerebral trauma. The expression of IGF-I in the two groups was detected by immunohistochemistry and western blot. Results The results of immunohistochemistry showed that the distribution of IGF-1 in neurons and glial cells was significantly increased in the temporal lobe of refractory epilepsy compared with the control group. Western blot results and immunohistochemistry results. Conclusion The increased expression of IGF-1 in brain tissue of patients with refractory epilepsy may be related to the protective mechanism of the brain. The presence of such neurotrophic factor helps to rescue the neurons damaged by epileptic seizures.