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采用Solexa高通量测序技术和蛋白定量方法,分析了碳化纤维素纸板制备的层状波纹碳(LCC)电极与石墨板(GP)电极在生物电化学系统运行过程形成的生物膜微生物群落结构和生物量差异.结果表明,LCC电极良好的生物电化学性能不仅取决于其良好的导电性能,还与其表面生物膜微生物群落结构有关;LCC电极表面生物膜微生物量高,且对产电微生物的富集效果好.LCC电极与GP电极表面生物膜分别得到16S rRNA基因V3区优化序列12643条和12837条,经97%相似度归并后获得的OTUs数量分别为2786和3130;α多样性分析显示,GP电极生物膜微生物多样性相对更丰富.Proteobacteria、Firmicutes和Bacteroidetes在两种电极生物膜中含量最为丰富,这3个门细菌序列数分别占总序列数的76%(LCC)和85%(GP).在属分类水平上,LCC电极生物膜由383个属的细菌构成,而GP电极生物膜则有456个属.深入分析电极生物膜微生物群落结构有助于进一步认识LCC电极提高生物电化学系统产电效率的机理.
Using Solexa high-throughput sequencing and protein quantification methods, the biofilm microbial community structure formed by laminar corrugated carbon (LCC) electrode and graphite plate (GP) electrode prepared by carbonized cellulose paper during biochemical electrochemical system operation was analyzed. The results showed that the good biochemical properties of LCC electrode depend not only on its good electrical conductivity but also on the structure of the biofilm community on the surface of LCC electrode. The results showed that 12643 and 12837 sequences of 16S rRNA V3 region were obtained from the biofilm on the surface of the electrode and GP electrode respectively. The numbers of OTUs obtained after the 97% similarity were 2786 and 3130 respectively. The microbial diversity of GP biofilm was relatively more abundant, while Proteobacteria, Firmicutes and Bacteroidetes were the most abundant biofilms in both electrodes, accounting for 76% (LCC) and 85% (GP) of the total number of sequences, respectively ) At the genus classification level, the LCC electrode biofilm consisted of 383 genera, while the GP electrode biofilm had 456 genera.Analysis of the electrode biomembrane microflora The structure helps to further understand the mechanism of LCC electrode to improve the power generation efficiency of bioelectrochemical system.