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目的观察地黄饮子对转tau基因果蝇AD模型PI3K/AKT/dTOR信号通路基因表达的影响,探讨其防治阿尔茨海默病的药物疗效和作用机制。方法将果蝇分为3组,即空白组、地黄饮子组、模型组。空白组为正常CS果蝇喂普通培养基,地黄饮子组为转tau基因果蝇喂2%浓度地黄饮子培养基,模型组为转tau基因果蝇喂普通培养基,各组均喂养5天。采用果蝇嗅觉记忆测试检测果蝇学习记忆能力,并运用荧光定量PCR法检测地黄饮子对转tau基因果蝇PI3K、AKT、dTOR mRNA表达的影响。结果 1嗅觉记忆测试中,各组进入有纱布障碍的离心管的果蝇数目变化趋势:空白组果蝇数量变化幅度最小,其次是地黄饮子组果蝇,模型组果蝇变化幅度最大。各组进入无纱布障碍的离心管的果蝇数目变化趋势:地黄饮子组果蝇前4 h进入离心管的数量变化速度较慢,4 h后数量变化速度明显高于其他组的果蝇;模型组果蝇进入离心管的数量整个过程比较缓慢,并且低于其他组果蝇;空白组的果蝇开始4 h进入离心管的数量变化较快并高于地黄饮子组果蝇,4 h后数量变化减慢明显并低于地黄饮子组果蝇。2与空白组比较,模型组和地黄饮子组PI3K、AKT、dTOR mRNA表达差异均有统计学意义(P<0.05)。地黄饮子组与模型组比较,PI3K、AKT、dTOR mRNA表达增加,差异有统计学意义(P<0.05)。结论地黄饮子可以改善转Tau基因果蝇的学习记忆能力,并上调转tau基因果蝇PI3K、AKT、dTOR mRNA的表达。
Objective To observe the effect of Rehmanniae Decoction on gene expression of PI3K / AKT / dTOR signaling pathway in AD transgenic Drosophila model of Drosophila melanogaster, and to explore its drug efficacy and mechanism of action in Alzheimer’s disease. Methods Drosophila were divided into 3 groups: blank group, Rehmanniae Decoction group and model group. The blank group was normal CS fruit fly fed normal medium, Rehmanniae group was fed with tau gene Drosophila feed 2% concentration of Rehmannia glutinosa medium, the model group fed tau gene Drosophila fed normal medium, each group were fed 5 day. Drosophila olfactory memory test was used to detect the learning and memory ability of Drosophila melanogaster. Fluorescent quantitative PCR was used to detect the effect of Dihuangyinzi on the expression of PI3K, AKT and dTOR mRNA in transgenic Drosophila melanogaster. Results In the olfactory memory test, the number of fruit flies entering into the centrifuge tube with gauze obstacles in each group tended to change: the least change in the number of fruit flies in the blank group followed by the fruit flies in the Rehmanniae group and the largest change in the model group. The change tendency of the number of fruit flies entering into the centrifuge tube without gauze barriers in each group: the quantity of the flies entering the centrifuge tube changed slowly 4 h before the fruit flies of D. officinalis, and the quantity change speed was obviously higher than that of the other groups after 4 h; In the model group, the number of flies entering the centrifuge tube was slower and lower than that of the other groups. The number of flies in the blank group changed significantly faster and higher than those in the Rehmanniae group after 4 h After the amount of change slowed significantly lower than the Rehmanniae group of fruit flies. Compared with the blank group, the expression of PI3K, AKT and dTOR mRNA in model group and Dihuang Yinzi group were significantly different (P <0.05). Compared with the model group, the expression of PI3K, AKT and dTOR mRNA increased in Rehmanniae Decoction group, with statistical significance (P <0.05). Conclusion Rehmanniae Decoction can improve the learning and memory ability of transgenic Drosophila melanogaster and up-regulate the expression of PI3K, AKT, dTOR mRNA in tau-transgenic Drosophila.