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失血性休克过程中,红细胞自身代谢与功能的变化会导致红细胞携氧-释氧能力的改变。本文对不同休克程度红细胞携氧-释氧能力的变化进行了监测,探讨失血性休克的危重程度与红细胞携氧-释氧能力之间的相互关系,并进行生理生化指标分析。本文采用健康SD大鼠20只(雌雄各10只),建立失血性休克模型,分别在初始、失血20%、30%和40%时采集红细胞,并利用血氧分析仪测定红细胞携氧-释氧热力学参数P50,效应时间Tc50、Tr50,同时检测了2,3-DPG、pH值、葡萄糖和乳酸脱氢酶的变化。实验结果表明:随着休克程度的加深,P50值不断增大,乳酸脱氢酶增加,而Tr50、pH值、葡萄糖均呈现减小的趋势,Tc50变化不明显;红细胞携氧能力相对稳定,而向组织释氧的能力仅略有增加,机体氧耗无法得到满足导致多器官衰竭。
During the process of hemorrhagic shock, the changes of erythrocyte own metabolism and function will lead to the change of redox oxygen-releasing capacity. In this paper, the changes of oxygen-releasing capacity of erythrocytes under different shock levels were monitored to investigate the relationship between the severity of hemorrhagic shock and oxygen-releasing capacity of erythrocytes, and the physiological and biochemical indexes were analyzed. In this paper, 20 healthy SD rats (10 males and 10 females) were used to establish hemorrhagic shock model. Erythrocytes were collected at initial, 20%, 30% and 40% of blood loss respectively. Oxygen thermodynamic parameters P50, Tc50, Tr50, and the changes of 2,3-DPG, pH, glucose and lactate dehydrogenase were also detected. The experimental results showed that with the deepening of shock, P50 value increased, lactate dehydrogenase increased, while Tr50, pH, glucose showed a decreasing trend, Tc50 changed insignificantly; erythrocyte oxygen carrying capacity was relatively stable, and The ability to release oxygen to the tissue is only marginally increased, and body oxygen consumption can not be met resulting in multiple organ failure.