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利用本室建立的小鼠骨髓内皮细胞株作传代培养 ,收集其无血清条件培养液 ,离心超滤 ,观察分子量(MW) >1 0 0 0 0组分及分子量 <1 0 0 0 0组分对红系生成的影响。结果表明 :分子量 >1 0 0 0 0组分对CFU E和BFU E有明显刺激作用 ,且在 2 %～ 6% (V/V ,下同 )范围内集落产率随浓度增加而增加 ;而分子量 <1 0 0 0 0组分对CFU E ,BFU E有明显抑制作用 ,且在 1 0 %～ 40 %范围内集落产率随浓度增加而降低
Using the mouse bone marrow endothelial cell line established in our laboratory for subculturing, the serum-free conditioned medium was collected, centrifuged and ultrafiltrated to observe the molecular weight (MW)> 1000 and the molecular weight <1000 The impact of red line generation. The results showed that the components with molecular weight> 1 000 were significantly stimulated by CFU E and BFU E, and the yield of colony increased with the increase of concentration in the range of 2% -6% (V / V, the same below) The fraction of molecular weight <1 0 0 0 0 had a significant inhibitory effect on CFU E and BFU E, and the yield of colony decreased from 10% to 40% with increasing concentration