一种新型Co~(2+)荧光探针的合成及其性能研究

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以8-羟基喹啉衍生物与2,4-二羟基苯甲醛为原料,合成了一种新型的离子荧光探针HQHD。该化合物结构通过红外、氢谱、碳谱以及高分辨质谱进行确认。当以V(乙腈)∶V(水)=1∶9缓冲溶液为溶剂时,荧光探针的性能研究表明,在16种常见金属离子和NH~+_4中,HQHD对Co~(2+)具有较好的专一识别能力,p H在6.98~8.6范围内对Co~(2+)的识别能力最强。当Co~(2+)与其他离子形成共存离子溶液时,并不影响HQHD对Co~(2+)的检测效果,说明配体HQHD具有较强的抗干扰能力。当Co~(2+)在0.5×10~(-5)~5×10~(-5)mol/L的浓度范围内,体系的荧光强度ΔF与Co~(2+)的浓度呈现良好的线性关系,线性拟合系数为0.982 6,检测限为9.77×10~(-8)mol/L。Job’s plot曲线表明,Co~(2+)与配体比为1∶1。 A novel ion-fluorescent probe, HQHD, was synthesized from 8-hydroxyquinoline and 2,4-dihydroxybenzaldehyde. The structure of this compound was confirmed by infrared, hydrogen, carbon and high-resolution mass spectra. Fluorescence probe performance studies showed that HQHD had a good effect on Co ~ (2+) in 16 kinds of common metal ions and NH ~ + _4 when V (acetonitrile): V (water) = 1: 9 buffer solution was used as solvent. Has better specific recognition ability, p H has the strongest ability to recognize Co 2+ in the range of 6.98 ~ 8.6. When Co 2+ forms coexisting ionic solution with other ions, it does not affect the detection of Co 2+ by HQHD, indicating that the ligand HQHD has strong anti-interference ability. When the concentration of Co 2+ is in the range of 0.5 × 10 -5 -5 × 10 -5 mol / L, the fluorescence intensity ΔF and the concentration of Co 2+ in the system exhibit good The linearity was 0.982 6 and the detection limit was 9.77 × 10 -8 mol / L. Job’s plot shows that the ratio of Co 2+ to ligand is 1: 1.
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