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取妊娠早期绒毛组织分离滋养层细胞，制成细胞悬液，离体培养。加入促性腺激素释放激素（ Ｇｎ Ｒ Ｈ）、人绒毛膜促性腺激素（ｈ Ｃ Ｇ）的分泌增加，单独加入雌三醇及孕酮，对 ｈ Ｃ Ｇ 的分泌无明显影响，若将 Ｇｎ Ｒ Ｈ 与雌三醇或与孕酮合用，则 Ｇｎ Ｒ Ｈ 所刺激的 ｈ Ｃ Ｇ 分泌明显被抑制。
Trophoblast cells were isolated from chorionic villi in early pregnancy to prepare cell suspension and cultured in vitro. The addition of gn R H and hCG increased secretion of estrogen and progesterone alone without any significant effect on the secretion of h C G. If Gn R H combined with estriol or with progesterone significantly inhibited hC G secretion stimulated by Gn R H.