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目的:探讨血小板活化因子(PAF)受体拮抗剂海风藤提取物对鼠脑缺血半暗带神经细胞凋亡的影响,并与传统的PAF受体拮抗剂银杏苦内酯相比较。方法:采用流式细胞分析和TUNEL方法,观察各实验组鼠脑缺血半暗带神经细胞的凋亡,并结合电镜观察缺血神经细胞超微结构的改变。结果:假手术组仅有极少量神经细胞凋亡;缺血90min再灌注12、24h缺血半暗带神经细胞凋亡明显增多;海风藤提取物、银杏苦内酯治疗组于相同时限内细胞凋亡较缺血/再灌组显著减少。结论:海风藤提取物与传统PAF受体拮抗剂银杏苦内酯均能明显减少缺血半暗带神经细胞的凋亡数量,减缓缺血区神经元损害,具有显著的脑保护作用。
OBJECTIVE: To investigate the effect of Piper kadsura Tropaeolol (PAF), a receptor antagonist, on neuronal apoptosis in ischemic penumbra in rats and compare with the traditional PAF receptor antagonist ginkgolide. Methods: Flow cytometry and TUNEL method were used to observe the apoptosis of neurons in the penumbra of ischemic penumbra of rats in each experimental group. The changes of the ultrastructure of ischemic neurons were observed by electron microscopy. Results: A very small amount of neuronal apoptosis was observed in the sham-operation group. Neuronal apoptosis in the ischemic penumbra at 12 and 24 h after reperfusion at 90 min was significantly increased. In the same time period, Apoptosis than ischemia / reperfusion group was significantly reduced. CONCLUSION: Both the extract of Piper kadsura and Ginkgolide, the traditional PAF receptor antagonist, can significantly reduce the number of apoptotic neurons in the ischemic penumbra and reduce the damage of neurons in the ischemic area, and have a significant brain protective effect.