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目的评价血清有机磷酸酯酶（ＰＥ）与肝纤维化的联系。方法用４０％ＣＣｌ４１ｍｌ／ｋｇ体重皮下注射，２次／周，共１４周诱发ＳＤ大鼠肝纤维化，分别于１，２，３，５，７，１０和１４周处死动物，测定血清和肝组织中ＰＥ、磷酸二酯酶Ⅰ（ＰＤＥⅠ）、单胺氧化酶（ＭＡＯ）、结合甘胆酸（ＣＧ）、β２＿微球蛋白（β２＿ＭＧ）、透明质酸（ＨＡ）和Ⅲ型前胶原（ＰＣⅢ）等。结果血清ＰＥ、ＰＤＥⅠ和ＣＧ于染毒后逐渐升高并与肝组织纤维化程度相关，ＡＫＰ和ＧＰＴ也升高，但呈不规则变化。ＭＡＯ，β２－ＭＧ，ＨＡ和ＰＣⅢ无变化。肝组织中羟脯氨酸含量增加，其它指标无变化。结论血清ＰＥ活性与肝纤维化程度密切相关，可用作诊断肝硬变
Objective To evaluate the relationship between serum organic phosphatase (PE) and hepatic fibrosis. Methods Liver fibrosis was induced by subcutaneous injection of 40% CCl41ml / kg body weight twice a week for 14 weeks in SD rats. Animals were killed at 1, 2, 3, 5, 7, 10 and 14 weeks respectively. Serum and liver PE, phosphodiesterase Ⅰ (PDEⅠ), MAO, CG, β2MG, HA, PCⅢ and so on. Results The levels of serum PE, PDE I and CG increased gradually after the exposure and were related to the degree of liver fibrosis. AKP and GPT also increased but irregularly changed. MAO, β2-MG, HA and PCⅢ no change. Liver hydroxyproline content increased, no change in other indicators. Conclusions Serum PE activity is closely related to the degree of liver fibrosis and can be used as a diagnosis of cirrhosis