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目的分析胸苷酸合成酶(Thymidylate synthetase,TYMS)基因在原发性肝癌中的表达及其临床意义。方法应用荧光定量PCR技术,对64例原发性肝癌TYMS基因进行扩增,免疫组化对照。结果在64例肝癌中有28例表达TYMS,占43.8%,拷贝数中位数M=3.55×104copies/mL,TYMS基因拷贝数对数(Log copies)与免疫组化分值有相关性,r=0.97,其中高拷贝(>1.0×105)表达有7例(10.9%),中拷贝((1.0~10)×104)表达有10例(15.6%),低拷贝(<10×103)表达有11例(17.2%),TYMS的表达与肿瘤大小、生存期(术后1年)、是否癌旁组织差异有统计学意义(P<0.05),而与病理类型、AFP水平、HBV-DNA差异无统计学意义(P>0.05)。结论荧光定量PCR技术可替代免疫组化方法检测TYMS基因的表达,TYMS基因在原发性肝癌有表达,对肿瘤生长和转移中有促进作用,可作为原发性肝癌的靶向治疗证据。
Objective To analyze the expression of thymidylate synthetase (TYMS) gene in primary hepatocellular carcinoma and its clinical significance. Methods 64 cases of primary hepatocellular carcinoma (TYCC) were amplified by fluorescence quantitative PCR and compared with immunohistochemistry. RESULTS: Among the 64 cases of hepatocellular carcinoma, 28 cases expressed TYMS, accounting for 43.8%. The median copy number was 3.55 × 104 copies / mL. There was a correlation between TYMS gene copy numbers and immunohistochemical scores. R = 0.97, of which 10 (15.6%) were expressed in high copy (> 1.0 × 105), 10.9% in medium copy (1.0-10 × 104), and low expressed There were 11 cases (17.2%) with TYMS expression and tumor size and survival (1 year after surgery), there was significant difference between adjacent tissues (P <0.05), but not with histological type, AFP level, The difference was not statistically significant (P> 0.05). Conclusion Fluorescent quantitative PCR can detect the expression of TYMS instead of immunohistochemistry. The expression of TYMS gene in primary hepatocarcinoma can promote tumor growth and metastasis, which can be used as evidence of targeted therapy for primary hepatocellular carcinoma.