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目的探讨HIV-1对感染者精子中IGF2和MEST基因甲基化及其表达的影响。方法采集HIV-1感染者精液,采用重亚硫酸盐测序法和实时荧光定量PCR(Real time-PCR)检测IGF2和MEST基因启动子甲基化状态及m RNA表达。结果与对照组比较,HIV实验组IGF2 m RNA表达水平略高于对照组,但两者差异比较无统计学意义(P=0.120);HIV实验组MEST m RNA表达水平低于对照组,两者差异比较有统计学意义(P=0.002);HIV实验组IGF2基因甲基化频率为(96.63±3.37)%,对照组甲基化频率为(96.02±1.84)%,两者比较无统计学差异意义(P=0.711);HIV实验组MEST基因甲基化频率为(1.29±1.04)%,对照组为(1.46±0.89)%,HIV实验组甲基化频率与对照组比较,差异无统计学意义(P=0.751)。结论在HIV实验组与对照组中检测IGF2、MEST启动子区甲基化状态均无差异。两组之间IGF2 m RNA也无差异,MEST m RNA表达在HIV实验组和对照组间存在显著差异,可能是HIV病人精液质量下降的一个原因。
Objective To investigate the effect of HIV-1 on the methylation and expression of IGF2 and MEST genes in sperm of infected persons. Methods The sera of HIV-1 infected persons were collected. The methylation status and m RNA expression of IGF2 and MEST gene promoter were detected by bisulfite sequencing and Real time-PCR. Results Compared with the control group, the expression level of IGF2 mRNA in HIV experimental group was slightly higher than that in control group, but there was no significant difference between the two groups (P = 0.120); MEST m RNA expression level in HIV experimental group was lower than that in control group The difference was statistically significant (P = 0.002). The methylation frequency of IGF2 gene was (96.63 ± 3.37)% in HIV group and 96.02 ± 1.84% in control group (P = 0.711). The methylation frequency of MEST gene was (1.29 ± 1.04)% in HIV experimental group and (1.46 ± 0.89)% in control group. There was no significant difference in methylation frequency between HIV experimental group and control group Meaning (P = 0.751). Conclusion There was no difference in the methylation status of IGF2 and MEST promoter between HIV experimental group and control group. There was no difference in IGF2m RNA between the two groups. MESTmRNA expression was significantly different between HIV experimental group and control group, which may be one of the reasons for the decline of semen quality in HIV patients.