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目的通过动物实验观察在胆道不同状态下逆行胆管造影对机体的影响．方法日本大耳白兔５１只随机分成６组，模拟胆道不同状态制作动物模型，即正常对照组５只，造影组５只，梗阻组７只，造影后梗阻组７只，梗阻后造影再梗阻组１３只，梗阻后造影解除梗阻组１４只，分别作逆行胆管造影．除造影组在注射造影剂后２４ｈ采集标本外，其它各组均在胆管测压后进行．测定门脉血中氧自由基、内毒素及胃动素，肝组织ｃＡＭＰ及脑组织β内啡肽含量．结果造影组与对照组相比各项指标无明显改变．梗阻状态下造影后继续梗阻组与对照组相比各项指标均有改变，表现在内毒素、氧自由基、β内啡肽、胃动素、肝脏ｃＡＭＰ明显升高．及时解除梗阻表现为氧自由基含量明显下降（Ｐ＜００５），内毒素含量呈下降趋势．结论生理状态下胆管造影对机体无任何不良影响．胆道梗阻状态下造影，机体的改变主要与梗阻本身有关，适时有效减压能使梗阻引起的损害逐渐好转．
Objective To observe the effect of retrograde cholangiography on the body under different conditions of biliary tract through animal experiments. Methods Fifty-one Japanese white rabbits were randomly divided into 6 groups. Animal models were made according to the different biliary conditions, that is, 5 normal control group, 5 angiography group, 7 obstruction group and 7 post-contrast obstruction group. Group 13, obstructive angiography lifted obstruction group 14, respectively, for retrograde cholangiography. In addition to the contrast group 24 hours after the injection of contrast agent samples, the other groups were performed after bile duct pressure measurement. Determination of portal blood oxygen free radicals, endotoxin and motilin, liver tissue cAMP and brain β endorphin content. Results Compared with the control group, the contrast group showed no significant change. Obstructive state continue to block after angiography compared with the control group indicators have changed, the performance of endotoxin, oxygen free radicals, β endorphin, motilin, liver cAMP was significantly higher. Timely release of obstruction showed a significant decrease in oxygen free radicals (P <0 05), endotoxin levels showed a downward trend. Conclusion Under physiological conditions, cholangiography has no adverse effect on the body. Biliary obstruction under the conditions of imaging, the body’s main changes and obstruction itself, timely and effective decompression can make the damage caused by obstruction gradually improved.