Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting

• 作 者: Ze Li Hai-Yuan Yang Ying Wang
• 机 构: JiangsuKeyLaboratoryofXenotransplantation,Peking-TsinghuaCenterforLifeSciences,ShenzhenXenotransplan
• 出 处: 生物医学研究杂志：英文版
• 发表日期: 2017年5期
• 关键词: CRISPR/Cas9 TRYPTOPHAN hydroxylase-2 gene serotonin Bama MINI PIGS CRISPR/Cas
• 基金项目: supported by a grant from the National Natural Science Foundation of China (No.81570402),,a grant from the Jiangsu Key Laboratory of Xenotransplantation (BM2012116),,grants from the Sanming Project of

Unbalanced brain serotonin （5-HT） levels have implications in various behavioral abnormalities andneuropsychiatric disorders. The biosynthesis of neuronal 5-HT is regulated by the rate-limiting enzyme, tryptophanhydroxylase-2 （TPH2）. In the present study,