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目的建立一种新的腰椎间孔外口植入髓核致坐骨神经痛大鼠模型。方法 54只雄性SD大鼠随机分为模型组、假手术组和空白组各18只。每组各取6只于术前1天及术后第1、3、5、7、10、14天行一般行为学观察和50%机械性撤足痛阈值(PWT)的测定。另各取6只分别于术后第3和7天检测术侧L5背根神经节中NOS和COX-2的表达。结果所有大鼠均未出现运动功能明显障碍。模型组50%PWT在术后各观察时点比术前、空白组和假手术组均明显减低(P<0.01)。模型组术侧L5背根神经节NOS和COX-2的阳性细胞数在术后第3和7天均较空白组和假手术组明显升高(P<0.01)。结论本研究建立的椎间孔外口植入髓核致坐骨神经痛大鼠模型创伤小,制作简便,不破坏关节结构,神经根性疼痛确实、持续、稳定。该模型为脊柱关节手法等保守治疗神经根刺激的机制研究提供了实验基础。
Objective To establish a new rat model of sciatica induced by implantation of nucleus pulposus in the lumbar intervertebral foramen. Methods Fifty-four male Sprague-Dawley rats were randomly divided into model group, sham-operated group and blank group. Six rabbits in each group were given routine behavioral observation and 50% mechanical withdrawal pain threshold (PWT) on day 1 and day 1, 3, 5, 7, 10 and 14 after operation. The other 6 rats were used to detect the expression of NOS and COX-2 in the L5 dorsal root ganglion on the 3rd and 7th day respectively. Results All rats showed no significant motor dysfunction. The 50% PWT in the model group was significantly lower than that in the preoperative, blank and sham operation groups (P <0.01). The numbers of positive cells of NOS and COX-2 in the L5 dorsal root ganglion in the model group were significantly higher than those in the blank group and sham operation group on the 3rd and 7th day after operation (P <0.01). CONCLUSION: The rat model of sciatica induced by the foramen inserted into the foramen of the foramen was established in this study. The traumatic model of the sciatica rat is small, easy to make, without damaging the structure of the joint. The radicular pain is indeed sustained and stable. The model provides the experimental basis for the study of the mechanism of nerve root stimulation by conservative treatment of spinal articular approach.