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目的观察rAd-p53、顺铂(DDP)单独及联合作用于人胃癌SGC7901细胞后,对肿瘤细胞增殖凋亡情况、KAI1/CD82蛋白表达情况的影响。方法 rAd-p53、DDP单独及联合作用于胃癌SGC7901细胞株24、48、72 h后,CCK-8法测定SGC7901细胞体外增殖活性,流式细胞术检测细胞凋亡率,免疫组化法检测KAI1/CD82蛋白表达情况。结果rAd-p53、DDP单独及联合作用于SGC7901细胞后,细胞增殖被抑制,并呈剂量和时间依赖性,且两药联合组细胞增殖抑制率明显高于单用组,与阴性对照组相比差异均有统计学意义(P<0.05);rAd-p53、DDP单独及两药联合作用SGC7901细胞48h后,细胞凋亡率为36.94%±0.78%、28.79%±2.37%,69.26%±0.63%;rAd-p53、DDP单独及两药联合均可上调KAI1/CD82蛋白表达,且两药联合组更明显。结论 rAd-p53、DDP单独及两药联合均可抑制胃癌SGC7901细胞的生长,诱导其凋亡,二者联合对胃癌细胞的抑制作用增强,rAd-p53可能通过上调KAI1/CD82的表达诱导SGC7901细胞凋亡,同时增强DDP的抗肿瘤作用。
Objective To observe the effect of rAd-p53 and cisplatin (DDP) on the proliferation and apoptosis of human gastric cancer cell line SGC7901 and the expression of KAI1 / CD82 protein. Methods The proliferation of SGC7901 cells was assessed by CCK-8 assay after 24, 48 and 72 hours of treatment with rAd-p53 and DDP alone and in combination. The apoptosis rate of SGC7901 cells was detected by flow cytometry. The expressions of KAI1 / CD82 protein expression. Results The proliferation of SGC7901 cells was inhibited by rAd-p53 and DDP alone and in a dose-and time-dependent manner. The inhibitory rates of cell proliferation in combination group were significantly higher than those in the control group (P <0.05). The apoptotic rates of SGC7901 cells treated with rAd-p53 and DDP alone or combined with the two drugs for 48 h were 36.94% ± 0.78%, 28.79% ± 2.37% and 69.26% ± 0.63% ; rAd-p53, DDP alone or in combination with both drugs can up-regulate KAI1 / CD82 protein expression, and the combination of the two drugs is more obvious. Conclusions Both rAd-p53 and DDP can inhibit the growth and induce the apoptosis of gastric cancer SGC7901 cells both alone and in combination. The combination of rAd-p53 and DDP can inhibit the growth of SGC7901 cells by increasing the expression of KAI1 / CD82 Apoptosis, while enhancing the anti-tumor effect of DDP.