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目的:通过比较肝癌及其癌旁组织的基因转录差异,探讨血管紧张素转化酶(angiotensin converting enzyme,ACE)对肝癌进程和预后的影响。方法:采用荧光定量PCR法对6例原发性肝细胞癌及癌旁组织中ACE、ACE2及其受体AT1(angiotensin recepor1,血管紧张素受体1)和MAS(血管紧张素1的7肽片段受体)的基因转录水平进行检测。结果:相对于癌旁组织,癌巢组织中ACE与ACE2的基因转录水平变化趋势一致,协同升高或降低;ACE/AT1的基因转录水平变化一致,协同升高或降低。结论:原发性肝细胞癌患者中,ACE/AT1受体与ACE2/MAS受体的调控作用是彼此制约,从而平衡的;而ACE可能是通过AT1起到调控作用的。
OBJECTIVE: To investigate the effect of angiotensin converting enzyme (ACE) on the progression and prognosis of hepatocellular carcinoma (HCC) by comparing the differences in gene transcription between HCC and its adjacent tissues. Methods: The expressions of ACE, ACE2 and angiotensin receptor 1 (angiotensin receptor 1) and MAS (angiotensin 1 7 peptide) in 6 cases of primary hepatocellular carcinoma and adjacent normal tissues were detected by real - Fragment receptor) gene transcription level was detected. Results: Compared with the adjacent non-cancerous tissues, the changes of gene transcription level of ACE and ACE2 in cancerous nests were consistent and synergistically increased or decreased. The gene transcription level of ACE / AT1 changed in a consistent manner and synergistically increased or decreased. CONCLUSIONS: The regulatory role of ACE / AT1 receptors and ACE2 / MAS receptors is restricted and balanced in patients with primary hepatocellular carcinoma, whereas ACE may play a regulatory role through AT1.