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采用高效液相色谱法,以主要有效成分五味子乙素为指标,测定护肝片中五味子乙素的含量。样品加样回收率为98.50%,RSD=1.79%。色谱条件为YWGC18柱(10μ,46×250mm),甲醇-水(72:28)为流动相,流速1ml/min。紫外检测波长254um。
The content of schisandrin B in Hugan Pills was determined by high performance liquid chromatography with the main active ingredient schizalrin B as an indicator. The sample recovery rate was 98.50% and RSD = 1.79%. Chromatographic conditions were YWGC18 column (10μ, 46×250mm), methanol-water (72:28) mobile phase, flow rate 1ml/min. UV detection wavelength 254um.