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目的探讨异常黑胆质成熟剂(ASM)对异常黑胆质型2型糖尿病(T2DM)大鼠的作用机制。方法采用核磁共振(NMR)技术结合模式识别和正交偏最小二乘判别(OPLS-DA)分析方法,寻找灌胃不同剂量ASM混悬液后异常黑胆质型T2DM大鼠尿液中的差异性代谢产物以及发生改变的代谢网络,分析ASM对异常黑胆质型T2DM大鼠尿液代谢的影响。结果灌胃ASM后,在异常黑胆质型T2DM大鼠尿液中下降的亮氨酸、苯丙氨酸、异亮氨酸以及柠檬酸、α-酮戊二酸、马尿酸、甲酸显著增加,同时在异常黑胆质型T2DM大鼠尿液中增多的β-葡萄糖和α-葡萄糖含量明显降低,与异常黑胆质型T2DM模型组比较差异有统计学意义(P<0.05)。结论 ASM对异常黑胆质型T2DM大鼠尿液代谢有显著影响,其作用机制可能是通过作用于异常黑胆质型T2DM引起的大鼠体内发生紊乱的糖代谢及氨基酸代谢而发挥治疗和预防异常黑胆质型T2DM的作用。
Objective To investigate the mechanism of abnormal savda (ASM) on abnormal Savda type 2 diabetes mellitus (T2DM) rats. Methods The nuclear magnetic resonance (NMR) technique combined with pattern recognition and Orthogonal Partial Least Squares (OPLS-DA) analysis was used to find the difference in urinalysis of abnormal Savda-type T2DM rats after intragastric administration of different doses of ASM suspension Sex metabolites, and altered metabolic networks, to analyze the effects of ASM on urinary metabolism in abnormal Savda-type T2DM rats. Results After administration of ASM, leucine, phenylalanine, isoleucine and citric acid, α-ketoglutarate, hippuric acid and formic acid decreased significantly in the urine of patients with abnormal savda type T2DM , While the levels of β-glucose and α-glucose in the urine of abnormal Savda T2DM rats were significantly lower than those in the abnormal savannah type T2DM rats (P <0.05). Conclusions ASM has a significant effect on urine metabolism in abnormal Savda-type T2DM rats. The possible mechanism is that ASM may play a role in the treatment and prevention of glucose metabolism and amino acid metabolism in rats induced by abnormal Savda-type T2DM The role of abnormal Savda T2DM.