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背景:束缚是研究心理应激的可靠方法。束缚应激可以通过下丘脑-垂体-肾上腺轴抑制T细胞免疫。目的:观察束缚应激状态下小鼠体内Th1/Th2平衡的改变以及荷瘤小鼠肿瘤生长情况,并试图发现心理应激对肿瘤细胞生长的影响。设计:以实验动物为研究对象的对照研究。单位:北京中医药大学基础医学院微生物学与免疫学教研室。材料:实验在北京中医药大学基础医学院免疫学实验室开展。选择鼠龄6～8周的昆明种小鼠为实验对象。购进后适应性饲养3d,称质量,并按体质量顺序编号,剔除体质量最大和最小的个体,再随机分入4个实验组中。分组后每组有小鼠16只(雌雄各8只),体质量18～20g。方法:取腹腔传代第7天的肿瘤细胞S180,用生理盐水洗涤后,用RP-MI1640细胞培养基调细胞密度至1×1010L-1,在单纯肿瘤组和肿瘤束缚组小鼠右腋皮下注射0.2mL/只。同时在正常对照组和单纯束缚组相同部位注射生理盐水0.2mL/只,接种后将单纯束缚组和肿瘤束缚组小鼠用特制小管限制活动,8h/d。10d后处死小鼠,剥取肿瘤和胸腺并分别称其质量,计算胸腺指数,分别采用四甲基偶氮唑盐比色法和丝裂原激活淋巴母细胞法检测脾T细胞增殖及产生Th1型细胞因子白细胞介素2、干扰素γ的能力,并取血清用酶联免疫吸附法检测Th2型细胞因子白细胞介素4、白细胞介素10的?
Background: Binding is a reliable way to study psychological stress. Stressed stress can suppress T cell immunity through the hypothalamus-pituitary-adrenal axis. OBJECTIVE: To observe the changes of Th1 / Th2 balance in mice and the tumor growth in tumor-bearing mice under restraint stress, and to find out the effect of psychological stress on tumor cell growth. Design: a controlled study of experimental animals. Unit: Department of Microbiology and Immunology, School of Basic Medicine, Beijing University of Chinese Medicine. Materials: The experiment was carried out in Immunology Laboratory of Basic Medical College of Beijing University of Chinese Medicine. Kunming mice aged 6 to 8 weeks were selected as experimental subjects. After the purchase of adaptive breeding 3d, said the quality, and according to the order of body mass numbers, remove the body mass of the largest and smallest individuals, and then randomly divided into 4 experimental groups. After grouping, mice in each group had 16 mice (8 males and 8 females) with a body mass of 18-20g. Methods: Tumor cells S180 of the 7th day after the passage of the abdominal cavity were obtained. After being washed with saline, the cell density was adjusted to 1 × 1010L-1 by RP-MI1640 cell culture medium. The mice in the simple tumor group and the tumor-binding group were subcutaneously injected with 0.2 mL / only. At the same time, 0.2 mL normal saline was injected into the same site of the normal control group and pure binding group. After inoculation, the mice in the restraint group and the tumor binding group were restricted with special tubules for 8 h / d. After 10 days, the mice were sacrificed and the tumor and thymus were stripped and their mass were respectively measured to calculate the thymus index. The proliferation of splenic T cells and the production of Th1 were detected by MTT colorimetric assay and mitogen-activated lymphoblastoid assay Type 2 cytokines interleukin - 2, IFN - γ, and serum samples were collected for detection of Th2 cytokines interleukin - 4, interleukin - 10 by enzyme - linked immunosorbent assay.