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在培养基中添加不同植物生长调节剂(生长素和细胞机动素),对美洲板粟愈伤组织的诱导以及植株再生进行了研究。将试管繁殖的小植株切为长0.5cm的小段作为外植体材料,以木本植物培养基加上3%的蔗糖和0.7%的琼脂作为基本培养基,再加上不同浓度的生长素或细胞机动素作为试验处理。生长素包括IBA,NAA,2,4-D和2,4,5-T;细胞机动素包括kinetin,CPPU,thidiazuron(TDZ)和zeatin。结果表明:外植体的植株再生和愈伤组织诱导百分率以及再生植株的形态发生以及愈伤组织的诱导量在不同的植物生长调节剂处理间和同一植物生长调节剂不同浓度的处理间都存在着显著的差异。促使外植体植株再生百分率最高的细胞机动素为TDZ,其次为CPPU,kinitin和zeatin;促使外植体愈伤组织诱导百分率最好的生长素为2,4,5-T,其次为2,4-D,IBA和NAA.
Different plant growth regulators (auxin and cytokinin) were added to the medium to study callus induction and plant regeneration in Panax pseudoginseng. The test tube-propagated plantlets were cut into 0.5 cm long pieces as explant material, with woody plant medium plus 3% sucrose and 0.7% agar as basic medium, supplemented with different concentrations of auxin or Cytokine is treated as a test. Auxins include IBA, NAA, 2,4-D and 2,4,5-T; cell motoks include kinetin, CPPU, thidiazuron (TDZ) and zeatin. The results showed that the percentage of plant regeneration and callus induction, the morphogenesis of regenerated plants and the callus induction amount of explants existed in different plant growth regulators and in different concentrations of plant growth regulators Significant differences. TDM was the most effective cell motan to induce explants to regenerate, followed by CPPU, kinitin and zeatin. The auxin with the best percentage of callus induction was 2,4,5-T, followed by 2, 4-D, IBA and NAA.