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采用核酸吸印杂交技术检测125例宫颈组织。宫颈糜烂、不典型增生、原位癌和浸润癌组织中HPV 16型及HPV 18型DNA相关序列阳性检出率分别是0%(0/10)、25%(1/4)、50%(1/2)和54%(59/109)。随宫颈病变加重HPV 16 DNA相关序列阳性率逐渐上升。临床以菜花型、结节型居多,为72.5%,HPV 16 DNA相关序列阳性率为72.9%。组织学检查,缺少凹空细胞的为61%,伴有凹空细胞的占39%。缺少凹空细胞的HPV16型DNA及HPV 18型相关序列阳性率为61%(36/59),符合Zur Hausen提出的HPV 16型及HPV 18型的组织学特征。鉴于HPV 18型同源序列检出率很低,HPV 33型或CHPV×1型同源序列也不高,说明宫颈癌的发生与HPV 16型同源序列感染关系更为密切。这种感染有着临床和病理学改变的特点,提示临床及病理医生在妇科检查及病理诊断中都应注意这些特点,以便早期诊断和早期治疗宫颈癌。
Detection of 125 cases of cervical tissue by nucleic acid blot hybridization. Cervical erosion, dysplasia, carcinoma in situ and invasive carcinoma of HPV 16 and HPV 18 DNA-related sequences were detected positive rates of 0% (0/10), 25% (1/4), 50% ( 1/2) and 54% (59/109). With the increase of cervical lesions HPV 16 DNA-related sequences positive rate gradually increased. Clinical cauliflower-type, nodular majority, was 72.5%, HPV 16 DNA-related sequence was 72.9% positive. Histological examination revealed 61% of the missing cells and 39% of the cells with concave cells. The positive rate of HPV16 DNA and HPV 18-related sequences lacking pits was 61% (36/59), in line with the histological features of HPV type 16 and HPV type 18 proposed by Zur Hausen. In view of the low detection rate of HPV 18 homologous sequences, HPV 33 or CHPV × 1 homologous sequences are not high, indicating that the occurrence of cervical cancer and HPV 16 homologous sequence infection more closely. This infection has the characteristics of clinical and pathological changes, suggesting that clinical and pathological doctors in gynecological examination and pathological diagnosis should pay attention to these features, so that early diagnosis and early treatment of cervical cancer.