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目的:考察生物相容性微乳(BME)抗H1N1流感病毒的作用,并采用网络药理学方法分析其作用机制。方法:运用四甲基偶氮唑蓝比色法(MTT)检测BME对A549细胞病变抑制率(ER),评价生物相容性微乳的抗病毒效果;利用网络药理学方法构建微乳对H1N1流感作用的网络,拟合微乳抗流感病毒的作用通路,分析其抗H1N1流感病毒的作用机制。结果:BME能够抵抗H1N1流感病毒,其作用呈现一定剂量依赖性,最大抗病毒效果ER为(67.573±2.628)%。与BME抗H1N1流感病毒最相关的4条基因本体相关信号传导通路分别为:MAPK通路、Toll-like受体信号通路、RIG-I-like受体信号通路和Jak-STAT信号通路。结论:BME具有显著抗H1N1流感病毒作用,其作用体现“多成分、多靶点、多通路”的作用特点,BME对H1N1流感的治疗不仅有直接作用,还存在间接的作用关系。
OBJECTIVE: To investigate the effect of biologically compatible microemulsion (BME) against H1N1 influenza virus and analyze the mechanism of action by using network pharmacology. Methods: MTT assay was used to detect the inhibitory rate (ER) of BME on A549 cells and the antiviral effect of biocompatible microemulsion was evaluated. The effect of microemulsion on H1N1 Influenza network, fitting the role of microemulsion anti-influenza virus pathway, analysis of its anti-H1N1 influenza virus mechanism of action. Results: BME was able to resist the H1N1 influenza virus in a dose-dependent manner with the maximum antiviral effect (ER) of 67.573 ± 2.628%. The four major oncogene-related signal transduction pathways that are most relevant to the BME anti-H1N1 influenza virus are the MAPK pathway, the Toll-like receptor signaling pathway, the RIG-I-like receptor signaling pathway, and the Jak-STAT signaling pathway. CONCLUSION: BME has significant anti-H1N1 influenza virus effect and its effect is characterized by “multi-component, multi-target, multi-pathway”. BME not only has a direct effect on the treatment of H1N1 influenza, but also indirectly.