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本文用神经毒药物6-OHDA注入大鼠右侧中脑被盖腹侧区,2周后用阿朴吗啡诱发旋转对受损动物进行筛选。结果发现,实验组125只大鼠中有52只鼠成为帕金森病模型,成功率为41.6%。用酪氨酸羟化酶(TH)抗体免疫组化法及荧光组化法对部分模型鼠的中脑及纹状体进行了组织学检查,证实受损侧黑质区多巴胺能神经元95%以上消失,纹状体TH阳性的黑质纹体纤维终末亦消失,但对侧存在。本实验为用脑移植治疗帕金森病的实验研究提供了可靠的动物模型。
In this article, the neurotoxic drug 6-OHDA was injected into the ventral region of the right midbrain of the rat, and 2 weeks later, the injured animals were screened by apomorphine-induced rotation. It was found that 52 out of 125 rats in the experimental group became Parkinson’s disease model, with a success rate of 41.6%. The histological examinations of the midbrain and striatum of some model rats were performed with tyrosine hydroxylase (TH) antibody immunohistochemistry and fluorescent histochemistry to confirm that 95% of the dopaminergic neurons in the substantia nigra region were damaged. The disappearance of the above, the striatum TH positive nigrostrialdehyde fiber terminal also disappeared, but the contralateral existence. This experiment provided a reliable animal model for the experimental study of brain transplantation for Parkinson’s disease.