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目的；为探讨局部脑缺血／再灌流中肿瘤坏死因子-α（TNF－α）的动态变化及其与白细胞浸润的关系。方法：采用大鼠大脑中动脉梗死模型，分别用免疫组织化学方法检测TNF－α、酶组化方法检测髓过氧化物酶（MPO）的动态变化，用图像分析进行定量分析。结果：TNF－α在缺血组（I）和缺血再灌流组（IR）0．5h表达增多，12h达高峰，与对照组比较差异非常显著（P＜0．01）．持续至120h；1组12h、IR组0．5hMPO明显增多，高峰在24h（P＜0．01）；I组TNF－α与APO有非常显著相关性（P＜0.01），IR组TNF－α与MPO有显著相关性（P＜0．05），证明局部脑缺血／再灌流损伤中TNF－α表达增多．其动态变化与损伤时间和局部白细胞浸润关系密切，结论：TNF－α是缺血性脑损伤的重要因素。
Objective To investigate the dynamic changes of tumor necrosis factor-α (TNF-α) and its relationship with leukocyte infiltration in focal cerebral ischemia / reperfusion. Methods: The model of middle cerebral artery occlusion in rats was established. The expression of TNF-α was detected by immunohistochemical method. The changes of myeloperoxidase (MPO) were detected by enzymatic histochemical method. Quantitative analysis was performed by image analysis. Results: The expression of TNF-α increased at 0.5 hour in ischemic group and IR group, reaching the peak at 12 hours, which was significantly different from the control group (P <0.01). (P <0.01). The level of TNF-α in the IR group was significantly higher than that in the APO group (P <0.01) And MPO (P <0.05), demonstrating the increased expression of TNF-α in focal cerebral ischemia / reperfusion injury. The dynamic changes were closely related to the injury time and the local leukocyte infiltration. Conclusion: TNF-α is an important factor of ischemic brain injury.