Optimization of Extraction Technology of Total Flavonoids from HERBA CENTIPEDAE by Orthogonal Test

• 作 者: Dengfeng ZOU Bingxiong PAN Suyi CHEN Aize XIE
• 机 构: Guilin Medical University,The First Affiliated Hospital of Guangxi Medical University,
• 出 处: Medicinal Plant
• 发表日期: 2016年Z1期
• 关键词: flavonoids controllable ultraviolet rutin references optimize purchased cough vo

[Objective] To optimize the extraction technology of total flavonoids from HERBA CENTIPEDAE by orthogonal test. [Methods]Content of total flavonoids was detected by ultraviolet spectrophotometry. With the extraction rate of total flavonoids as the index,effects of enzymolysis time,pH value,cellulase concentration and enzymolysis temperature were researched. [Results] The optimal extraction technology was as follows: pH = 5,2. 5 h enzymolysis time,0. 3 mg / mL cellulase concentration and 37 ℃ enzymolysis temperature. Under this condition,the extraction rate of total flavonoids was 2. 814 7%. [Conclusions] This method was stable,easy and controllable,and provided references for the further development and utilization of HERBA CENTIPEDAE.

[Objective] To optimize the extraction technology of total flavonoids from HERBA CENTIPEDAE by orthogonal test. [Methods] Content of total flavonoids was detected by ultraviolet spectrophotometry. With the extraction rate of total flavonoids as the index, effects of enzymolysis time, pH value, cellulase concentration and enzymolysis temperature were researched. [Results] The optimal extraction technology was as follows: pH = 5,2. 5 h enzymolysis time, 0.3 mg / mL cellulase concentration and 37 ° C enzymolysis temperature. Under this condition, the extraction rate of total flavonoids was 2. 814 7%. [Conclusions] This method was stable, easy and controllable, and provided for further development and utilization of HERBA CENTIPEDAE.