【摘 要】
：
A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China.The objective of the present study was to evaluate the cross-protective efficacy of the live vaccine TJM-F92 in
【机 构】
：
Sinovet(Beijing)Biotechnology Co., Ltd, Beijing 100085, China;State Key Laboratory for Molecular Bio
论文部分内容阅读
A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China.The objective of the present study was to evaluate the cross-protective efficacy of the live vaccine TJM-F92 in young pigs against challenge with a NADC30-1ike strain,HN201605.Twenty-five PRRSV-and antibody-free pigs were randomly divided into the following five groups:Vac/ChA,Unvac/ChA,Vac/ChB,Unvac/ChB and the mock.The pigs in groups Vac/ChA and Vac/ChB were inoculated intramuscularly with 1 mL TJM-F92 (105.0 TCID50/mL).At 28 days post vaccination (0 days post challenge),groups Vac/ChA and Unvac/ChA were inoculated intranasally with 104.5 TCIDs0/mL PRRSV strain TJ F3 (2 mL/pig),while groups Vac/ChB and Unvac/ChB were inoculated,using the same route,with the same dose of the NADC30-1ike strain HN201605 F3.Protective effects of the PRRSV strain were observed in all pigs in the Vac/ChA and Vac/ChB groups.Neither high fever nor signs of clinical disease were observed through the experiment in these groups,whereas pigs in Unvac/ChA group exhibited serious clinical symptoms,pathological lesions,and weight loss.In Unvac/ChB group,pigs developed milder clinical symptoms,which demonstrated that the NADC30-like strain HN201605 had moderate pathogenicity.The results suggest that the MLV vaccine strain TJM-F92 is an effective and safe vaccine candidate for use in China.
其他文献
HIV-1 Rev is an accessory protein that plays a key role in nuclear exportation,stabilization,and translation of the viral mRNAs.Rev of HIV-1 clade BC often shows a truncation of 16 AAs due to a premature stop codon at residue 101.This stop codon presents
The Lorne Infection and Immunity Conference is one of five scientific meetings held during each month of February at the Cumberland resort in the picturesque seaside town of Lorne,on the Great Ocean Road in Victoria (Australia).
Kaposi\'s sarcoma-associated herpesvirus (KSHV),also known as human herpesvirus-8 (HHV-8),is etiologically linked to the development of Kaposi\'s sarcoma,primary effusion lymphoma,and multicentric Castleman\'s disease.These malignancies often occur
Enterovirus 71 (EV71),a major causative agent of hand,foot,and mouth disease,has caused periodic infection outbreaks in children in the Asia-Pacific region.In order to describe the largely unknown life cycle of EV71,the molecular basis of its virus-host i
Annual influenza B virus epidemics and outbreaks cause severe influenza diseases in humans and pose a threat to public health.China is an important epidemic area of influenza B viruses.However,the spatial,temporal transmission pathways and the demography
Dengue virus (DENV) infection is a worldwide public health threat.To date,the knowledge about the pathogenesis and progression of DENV infection is still limited.Combining global profiling based on proteomic analysis together with functional verification
The Crimean-Congo hemorrhagic fever virus (CCHFV),a member of the genus Orthonairovirus and family Nairoviridae,is transmitted by ticks and causes severe hemorrhagic disease in humans.To study the epidemiology of CCHFV in different ecosystems in Xinjiang,
Interferon,a natural protein that is produced by a variety of cells during viral infection,activates the transcription of multiple functional genes in cells,regulates synergy among various signaling pathways,and mediates many biological functions such as
Cancer cell lines have been used widely in cancer biology,and as biological or functional cell systems in many biomedical research fields.These cells are usually defective for many normal activities or functions due to significant genetic and epigenetic c
Rabies is a zoonotic disease that still causes 59,000 human deaths each year,and rabies vaccine is the most effective way to control the disease.Our previous studies suggested that the maturation of DC plays an important role in enhancing the immunogenici