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目的:探讨连翘叶苷类成分(glycosides in forsythia suspensa leaves,FSG)对力竭游泳小鼠心肌和骨骼肌的保护作用。方法:健康雄性ICR小鼠随机等分为安静对照组、运动对照组、运动+FSG大剂量组和运动+FSG小剂量组,每组12只。给药组小鼠以相应剂量FSG灌胃20天,在最后一次给药后22h,运动组小鼠进行一次力竭游泳,并记录力竭时间。用分光光度法测定力竭运动后各组小鼠心肌和骨骼肌MDA含量和T-SOD、CuZn-SOD活性。用免疫组化法(SABC法)测定小鼠心肌和骨骼肌细胞CuZn-SOD蛋白表达。电子显微镜观察各组小鼠心肌和骨骼肌细胞超微结构。结果:FSG组的MDA含量均低于运动对照组,T-SOD和CuZn-SOD活性均高于运动对照组,CuZn-SOD表达也呈现同一趋势。电镜下可见运动对照组小鼠心肌和骨骼肌细胞、线粒体肿胀,空泡样变,闰盘严重损伤,肌原纤维排列杂乱,细胞核核质溶解和肌浆网扩张。而FSG大剂量组的损伤程度明显减轻,与安静对照组接近。结果表明:FSG对力竭游泳小鼠心肌和骨骼肌有一定保护作用。
Objective: To investigate the protective effect of forsythia suspensa leaves (FSG) on myocardium and skeletal muscle of exhausted swimming mice. Methods: Healthy male ICR mice were equally divided into quiet control group, exercise control group, exercise + FSG high dose group and exercise + FSG low dose group, 12 rats in each group. Mice in the drug-treated group were given gavage for 20 days at the corresponding dose of FSG. At 22 hours after the last administration, the mice in the exercise group were subjected to an exhaustive swimming and the exhaustion time was recorded. The myocardial MDA and T-SOD, CuZn-SOD activity in myocardium and skeletal muscle of mice in each group were determined by spectrophotometry. The protein expression of CuZn-SOD in myocardium and skeletal muscle of mice was detected by immunohistochemistry (SABC method). The ultrastructure of myocardial and skeletal muscle cells in each group was observed by electron microscope. Results: The content of MDA in FSG group was lower than that in the control group. The activities of T-SOD and CuZn-SOD were higher than those in the control group. The expression of CuZn-SOD also showed the same trend. Under the electron microscope, the myocardium and skeletal muscle cells of mice in the exercise control group showed mitochondrial swelling, vacuolar degeneration, serious damage of intercalated discs, disorganized myofibrils, lysis of nuclear and nuclear sarcoplasmic reticulum. In the FSG high-dose group, the degree of injury was significantly reduced, which was close to the quiet control group. The results showed that: FSG had a protective effect on myocardium and skeletal muscle of exhausted swimming mice.