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目的 :观察噪声暴露于对耳蜗外淋巴液中氨基酸浓度的影响。 方法 :安静组和噪声组豚鼠分别暴露于安静环境 (4 0d B SPL )和白噪声 (115 d B SPL ) 2 h,抽取两组动物耳蜗外淋巴液 ,高效液相色谱 (HPL C)荧光分光检测分析氨基酸浓度。 结果 :安静组动物耳蜗外淋巴液中含有 14种氨基酸 ,与正常哺乳动物脑脊液氨基酸组成和含量十分接近。耳蜗外淋巴液中谷氨酸含量安静组为 (6 .6± 0 .2 )μmol/ L ,而噪声组为 (10 .3± 1.1)μm ol/ L ,后者比前者高 5 5 %,P<0 .0 1。 结论 :噪声暴露可使耳蜗外淋巴中谷氨酸浓度明显提高 ,推测这种提高可能和噪声导致毛细胞过度释放谷氨酸和谷氨酸转运体不能正常运转有关。
Objective: To observe the effect of noise exposure on amino acid concentration in extralymphatic cochlea. Methods: Quiet and noise guinea pigs were exposed to quiet environment (40 d SPL) and white noise (115 d B SPL) for 2 h respectively. Two groups of animals were examined by cochlear perilymph and high performance liquid chromatography (HPL C) Detection and analysis of amino acid concentration. Results: 14 kinds of amino acids were found in the perilymph of the quiet animals, which was similar to that of normal mammalian cerebrospinal fluid. The level of glutamate in cochlear extranodal fluid was (6.6 ± 0.2) μmol / L in quiet group and (10.3 ± 1.1) μmol / L in noise group, while the latter was 55% higher than the former <0 .0 1. CONCLUSIONS: Exposure to noise can significantly increase glutamate concentration in the extralymphatic cochlea, suggesting that this increase may be related to the noise-induced over-release of glutamate and glutamate transporters.