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目的:探讨维生缺B_1缺乏(TD)对成年小鼠室管膜下区(SVZ)神经发生的影响。方法:TD小鼠模型按TD喂食时间分为TD7、TD14和TD21 d组,正常对照组(Con)小鼠则按标准喂食及时间亦分为Con 7、Con 14和Con 21 d组(每组n=6)。小鼠处死前5 d腹腔给予5-溴脱氧尿嘧啶核苷(BrdU)。用免疫荧光组化染色分别检测SVZ的BrdU和微管相关蛋白(Dcx)阳性细胞的分布和数目。结果:正常组的BrdU阳性细胞和Dcx阳性细胞在SVZ均匀分布在侧脑室背外侧角,侧脑室外侧壁亦有少量分布,TD14和TD21 d组两类阳性细胞的减少主要为侧脑室背外侧角。在TD14和TD21 d组,室管膜下区BrdU标记阳性细胞数(像素/mm~2值分别为74.97±7.75和67.27±10.91)明显低于对照组(像素/mm~2值分别为122.30±8.86和122.99±5.33),Dcx标记阳性细胞数(像素/mm~2值分别为1 485.98±163.21和935.73±273.66)也明显低于对照组(像素/mm~2值分别2 359±155.68和2 355±100.75)。结论:TD可阻碍成年小鼠SVZ的神经发生。
Objective: To investigate the effects of vitamin B deficiency (TD) on neurogenesis in subventricular zone (SVZ) of adult mice. Methods: The TD mice were divided into TD7, TD14 and TD21 d groups according to TD feeding time. Con mice were divided into Con 7, Con 14 and Con 21 d groups according to standard feeding and time n = 6). 5-bromodeoxyuridine (BrdU) was intraperitoneally administered to mice 5 days before sacrifice. Immunofluorescence staining was used to detect the distribution and number of BrdU and microtubule-associated protein (Dcx) positive cells in SVZ. Results: In normal group, BrdU-positive cells and Dcx-positive cells were evenly distributed in the dorsolateral horn of lateral ventricle in SVZ with a small amount of distribution in the lateral lateral ventricle. The decrease of the two types of positive cells in TD14 and TD21 d were mainly the dorsolateral angle . In TD14 and TD21 group, the number of BrdU positive cells (74.97 ± 7.75 and 67.27 ± 10.91 respectively) in subventricular zone was significantly lower than that in control group (the pixel / mm ~ 2 values were 122.30 ± 8.86 and 122.99 ± 5.33). The number of Dcx positive cells (1 485.98 ± 163.21 and 935.73 ± 273.66 respectively) was also significantly lower than that of the control group (2 359 ± 155.68 and 2 355 ± 100.75). Conclusion: TD can hinder the neurogenesis of SVZ in adult mice.