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目的了解四川省德阳市埃柯病毒6型(echovirus 6,E6)的基因特征。方法对2013年德阳市手足口病咽拭子标本的病毒分离阳性株进行核苷酸测序,获取的E6毒株序列与世界各地的E6参考株序列构建进化树并分析基因特征。结果4株E6分离株中,3株VP1区核苷酸序列一致,与另1株仅有1个核苷酸差异。同E6原型株(D’Amori)比较,核苷酸一致性≥77.0%。进化树分析显示,德阳市E6分离株为C2基因亚型,与部分山东分离株聚集在同一进化分支上。结论德阳市E6为C2基因亚型。其中3份标本来自同一地区的不同月份,提示E6在此地持续传播。
Objective To understand the genetic characteristics of echovirus 6 (E6) in Deyang City, Sichuan Province. Methods Nucleotide sequencing was performed on the virus-positive isolates of throat swab specimens of hand-foot-mouth disease in Deyang city in 2013. E6 strain sequences were obtained and phylogenetic trees of E6 reference strains were constructed from all over the world and the gene characteristics were analyzed. Results Among the four E6 isolates, the nucleotide sequences of the three VP1 regions were identical and only one nucleotide difference from the other strain. Nucleotide identity> 77.0% compared to the E6 prototype strain (D’Amori). Phylogenetic tree analysis showed that the E6 isolates from Deyang City were subtypes of C2 gene and clustered with some Shandong isolates on the same evolutionary branch. Conclusion Deyang City E6 is C2 subtype. Three of the specimens came from different months in the same area, suggesting that E6 continues to spread here.