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利用反向ＰＣＲ法，缺失人ＩＬ－８ｃＤＮＡ内部ｂｐ序列，构建得到ＩＬ－８突变内标分子．用 已知量的该内标分子，通过竞争性ＲＴ－ＰＣＲ法、检测支气管哮喘患者外周血ＩＬ－８基因的表达情 况，发现患者ＩＬ－８ｍＲＮＡ的表达水平明显高于正常人对照组．这一结果初步表明，利用竞争 性ＲＴ－ＰＣＲ技术对支气管哮喘息者进行早期基因诊断，具有一定的可行性。
Using reverse PCR, the deletion of human IL-8cDNA bp sequence, build IL-8 mutant internal standard molecules. Using a known amount of the internal standard molecule, the expression of IL-8 mRNA in peripheral blood of patients with bronchial asthma was detected by competitive RT-PCR. The expression level of IL-8 mRNA in patients with bronchial asthma was significantly higher than that in normal controls. This result initially shows that the use of competitive RT-PCR technology for early diagnosis of bronchial asthma who have a certain degree of feasibility.