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目的:研究愤怒应激对D-半乳糖(D-gal)诱导的脑老化模型大鼠认知功能的影响及机制。方法:将Wistar大鼠随机分成正常对照组、愤怒模型组、D-gal模型组愤怒+D-gal模型组及攻击鼠,颈后部sc 1%D-gal生理盐水溶液,注射容量为10 mL.kg-1.d-1,正常对照组注射同体积生理盐水,连续注射6周。愤怒组、愤怒D-gal组,从第三周始进行愤怒刺激实验,把愤怒模型组大鼠单独放入鼠笼中,随机放进一只入侵鼠,用纱布包裹止血钳钳夹入侵鼠尾,入侵鼠对模型组大鼠发起攻击,双方互相撕咬对峙,每天刺激时间为20 min,连续4周。第37天开始Morris水迷宫实验测试,6 d后处死解剖大鼠,收集脑组织进行超氧化物歧化酶(SOD)、丙二醛(MDA)、脂褐素(LPF)检测;同时对大脑皮质及海马区进行病理学观察。结果:与正常对照组相比,愤怒模型组、D-gal模型组、愤怒+D-gal模型组脑SOD活性明显降低(P<0.01),MDA,LPF含量明显升高(P<0.01),空间学习记忆能力明显降低(P<0.05);行为活动和学习记忆功能减退差异显著(P<0.05,P<0.01);病理学检测结果显示,与正常对照组相比,愤怒模型组、D-gal模型组、愤怒+D-gal模型组大脑皮质和海马区均出现不同程度的病理变化,其中愤怒+D-gal模型组病变最为明显。结论:愤怒应激对D-gal诱导的脑老化模型大鼠的行为活动和认知功能具有减退作用。
Objective: To investigate the effect and mechanism of anger stress on cognitive function in D-gal-induced brain aging model rats. METHODS: Wistar rats were randomly divided into normal control group, anger model group, D-gal model group and anger + D-gal model group and challenge rats, the posterior cervical sc 1% D-gal saline solution with an injection volume of 10 mL .kg-1.d-1, normal control group injected with the same volume of saline, continuous injection of 6 weeks. Angry group, angry D-gal group, starting from the third week of anger stimulation experiment, the anger model group rats were placed in squirrels alone, randomly into an invading mouse, wrapped with gauze clamp forceps invade the tail , Invaded rats on the model group rats attack, the two sides bite each other confrontation, stimulation time every day for 20 min, for 4 weeks. Morris water maze test was started on the 37th day. After 6 days, the rats were sacrificed and the brain tissues were harvested for the detection of superoxide dismutase (SOD), malondialdehyde (MDA) and lipofuscin (LPF) And hippocampus area pathological observation. Results: Compared with the normal control group, the SOD activity in the anger model group, the D-gal model group and the anger + D-gal model group were significantly decreased (P <0.01), MDA and LPF contents were significantly increased The spatial learning and memory abilities decreased significantly (P <0.05), and there was significant difference between behavioral activity and learning and memory abilities (P <0.05, P <0.01). The pathological results showed that compared with the normal control group, In the model group of gal, the pathological changes of cerebral cortex and hippocampus were observed in the model of anger + D-gal, and the pathological changes were most obvious in the group of anger + D-gal. Conclusion: Anger stress has a diminished effect on behavioral activity and cognitive function in D-gal-induced brain aging rats.