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以聚乙二醇-聚L-乳酸为载体材料,用高压静电纺丝法制得包载厚朴酚的纤维毡。扫描电镜和Photoshop 5.0软件测定结果表明得到了表面光滑、直径较均一的纤维;广角X-射线衍射结果表明载药纤维表面无药物结晶。采用HPLC法测定了载药纤维毡中厚朴酚在含或不含蛋白酶K的磷酸盐缓冲液中的累积释放率。结果表明,当介质中加入蛋白酶K(含量为2.5 g/ml)后,厚朴酚的释放速率即明显加快。酶浓度由2.5 g/ml升至10 g/ml时,厚朴酚释放速率继续增加,但增幅较小。介质中无蛋白酶K存在时,将释放曲线分段后用Higuchi方程拟合效果较好;有蛋白酶K存在时,药物释放曲线在平台期前呈现一级释放动力学形式。
Polyethylene glycol-poly-L-lactic acid was used as carrier material to prepare honokiol-containing fiber mat by high-pressure electrospinning. Scanning electron microscopy and Photoshop 5.0 software results showed that the surface was smooth, more uniform diameter fibers; wide angle X-ray diffraction results showed that drug-loaded fiber surface without drug crystallization. The cumulative release rate of honokiol in drug-loaded fiber mats in phosphate buffered saline with or without proteinase K was determined by HPLC. The results showed that the release rate of magnolol was obviously accelerated when adding proteinase K (2.5 g / ml) to the medium. When the enzyme concentration increased from 2.5 g / ml to 10 g / ml, the release rate of magnolol continued to increase, but with a small increase. In the absence of proteinase K, the release curve was better fitted by Higuchi equation. In the presence of proteinase K, the drug release curve showed a first-order kinetic form before plateau.